Grand father was Blackie mother albino, gave birth to 6 kids three brownie same pattern of patch with slight variation, two albinos n one Blackie photocopy of father. The above kid was born on 31st of Jan this year her mother is an albino n father Brownie how come?
Final vaccine for AIDS--This is a live recombinant vaccine, if we are able to find out the gene for the KEY enzymes by removing those genes we cud prepare vaccines..
So by producing virus with
1.DNA that codes for the protein part structural or enzyme missing, one cud prevent further infection n prepare a vaccine after checking that further cycles did not take place.
2.DNA for the enz needed for RNA n DNA synthesis missing this cud act as a perfect live recombinant vaccine.
Earlier thought--Vaccine for AIDS--Create a virus with some genes removed, one cud identify the genes for Gp120 n remove that gene, the virus will enter the body but will not be able to enter the T lymphocytes n further multiplication will take place but the next progeny will lack Gp120 n will not be able to infect the cells further. Bcoz the virus will be in the body antibodies will be formed against them, but they will not cause any harm n these antibodies formed cud protect from a future infection, this will act as the antigen but will not infect the cell.
But found out that HIV grows in T cells, Bcells, microphage n neural cells, still for the preperation of AIDS vaccine we need not culture the above cells and grow the virus in them but should grow in other cells as well.
Turning point which made tissue culture the most important method of cultivation of viruses was demonstration by Endees, Weller n Robbins (1949) that Poliovirus, till then considered strictl;y a neurotrophic virus, cud be grown in tissue culture of non neural origin.Since then almost every human virus has been grown in tissue culture.
This also means that different viruses cud be grown in different animals like the rabies virus which grows in so many animals there is a chance that different animals cud harbour many viruses, chick n duck embryo is used to culture so many viruses, these viruses cud grow in hens n ducks as well.
The reactogenicity of killed vaccines has been attempted to be reduced by purification of the viruses. Adv rkns may be reduced only by the use of subunit vaccines in which the virus is split by detergents or other chemicals n only relevant antigens incorporated in the vaccine.
Live vaccines are heat labile n have to be kept under refrigeration .If the temp rises the vaccine will be destroyed n will lead to vaccine failure.
To prepare a culture medium of Bcells thought about irradiating an egg and then innoculating a lymphoid stem cell which cud produce T and B lymphocytes where the HIV cud be cultured.
THE points collected from the above material makes one realise that AIDS virus cud grow in other cells as well n Gp 120 cud not be essential for cell entry so thought about a different vaccine
The virus is DNA packed with a protein cover, the protein acts as the antigen, this will act as the vaccine.
This will lead to preperation of killed vaccine, for eg cud try growing the AIDS viorus in the medium mentioned above n then
prepare a killed virus in the following manner--
Methods of preparing different types of vaccines--
Killed vaccine prepared by inactivating viruses with heat, phenol, formalin or Beta propiolactone.
Live attenuated vaccine - Virus attenuated by serial passage in eggs , culture mediums.
Live recombinant vaccines.
Rabies virus multiplies in human brain suggests that the nerve cells multiply was generally thought they don't so take enough proteins, bcomplex and milk.This cud help in new nerve cell formation,same applies to a patient with brain haemorrhage.
All this began yesterday afternoon when my 8 bunny princess spread out on my bed n me on the floor on the dari n began with the egg story...
Why is it that one virus that infects a bacteria does not infect man and a Virus infects only a particular type of cell?
Thought about the answer Proteins of the virus binds with certain receptors present on only some cells so the affinity of some virus for some cells n not all. If we inject the DNA of a virus into any dividing cell will multiply with it hopefully:").
When a virus is introduced into an egg, will not multiply if temperature not raised bcoz embryo in the egg multiplies at a higher temperature n till the DNA multiiplication takes place the virus will not multiply, the embryo in the egg is alive n respiring, even in a dead body anaerobic respiration takes place leading to lactic acidosis, have already told about storing a dead body at freezing temp and then giving a DC current to reactivate the heart...The zygote's development begins inside the bird's body itself.
IF the virus is introduced in a system where there is slow cell multiplication viral multiplication will also be slow, so keep AIDS, Hep B and cancer patients at a cooler temperature, earlier TB sanatariums were built at hill stations same cud apply for viral infections. Also give antifolates that stop DNA synthesis n cell multiplication .
If U wish to introduce melanin gene into farm eggs that yeild albino chicks then depending on the viral multiplication the melanin gene will be introduced n one may get black n white kids.
In Lytic cycle the viruses are released after multiplication in a cell n then they enter another cell. From this it is clear that the virus after being released from the cells stay in a state of just anatomy no physiology in the environment so it is not necessary that virus growing in living tissue is the only source of viral infection, so keep ur environment dust free as well.
If u wish to decrease the incubation period of the life in the egg introduce thyroxine that increases the Basal metabolic rate n will cause rapid cell multiplication n growth in the embryo.During fever the cell rkns get hastened n oxygen requirement also increases the activity in all the cells of the body, this affects the brain as well which manifests as a febrile seizure. Similarly during thyrotoxicosis the BMR is increased so if an egg's growth is to be hastened maintain warmth thruout n see if small amounts of thyroxine works.
Culture skin cells in an egg, birds with higher incubation period better, take these cells n apply to the bare area of a burn injury or a wound just like skin grafting.
Similar to above introduce Colony Forming Unit --E this will produce RBCs, then add the RBCs to normal saline and transfuse slowly, small amounts of egg components should not do much harm, check this, if fear of infection then kill all the bacteria will antibiotics in the culture media itself.
True meiosis lacking in Haplodiploidy eg in Apis indica --honey bee,
Meiosis totally lacking in diploid parthenogenesis--Here diploid eggs produced with one polar body only.
Parthenogenesis produces clones.
Primary oocyte if induced to get activated will give rise to a diploid human clone.